Quality control strains

Escherichia coli

PTCC No: 
1338
Other collection No. :: 
ATCC 10536, DSM 682, NCIMB 8879, NCTC 10418, CCUG 3274
Category: 
Quality control strains
Reference strains
Culture condition: 
37 °C
Information: 
<-ATCC <- American Cyanamid Co. Assay of chloramphenicol, colistin colimycin, spectinomycin actinospectacin, tetracycline and rifamycin. Quality control strain. Used for sterility testing, bactericides testing, pharmaceutical testing and validation control.
Literature: 

Antibiotic assays (European Pharmacopoeia Commission Microbiological assay of antibiotics. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.7.2, 1997; British Pharmacopoeia Commission Biological assay of antibiotics. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XIV A, 2003; U.S. Pharmacopeia General Chapters; <81> Antibiotics-Microbial Assays. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011.) Tetracyclin assay (U.S. Pharmacopeia USP Monographs: Tetracycline Hydrochloride and Novobiocin Sodium Tablets. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011). Disinfectants assays (Chemical disinfectants and antiseptics --- Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas --- Test method and requirements (phase 2, step 1). London, UK:British Standards Institution;British Standard BS EN 1276:1997, 1997; Chemical disinfectants and antiseptics - Preservation of test organisms used for the determination of bactericidal, sporicidal and fungicidal activity. London, UK:British Standards Institution;British Standard BS EN 12353:2006, Chemical disinfectants and antiseptics --- Quantitative non-porous surface test for the evaluation of bactericidal and/or fungicidal activity of chemical disinfectants used in food, industrial, domestic and institutional areas --- Test method and requirements without mechanical action (phase 2/step 2). London, UK:British Standards Institution;British Standard BS EN 13697:2001.; Chemical disinfectants and antiseptics --- Application of European Standards for chemical disinfectants and antiseptics. London, UK:British Standards Institution;British Standard BS EN 14885:2006). Cleanroom biocontamination control (Cleanrooms and associated controlled environments--Biocontamination control--Part 1: General principles and methods. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 14698-1:2003.; Cleanrooms and associated controlled environments --- Biocontamination control --- Part 1: General principles and methods. London, UK:British Standards Institution;British Standard BS EN ISO 14698:2003). Sterility testing (Bowman FW, et al. Collaborative study of aerobic media for sterility testing by membrane filtration. J. Pharm. Sci. 60: 1087-1088, 1971. PubMed: 5000479)

Supplied as: 
Freeze-dried
Risk group: 
2

Clostridium sporogenes

PTCC No: 
1651
Other collection No. :: 
ATCC 19404, NCIMB 532, NCTC 532, DSM 1664, WDCM 00008
Category: 
Quality control strains
Reference strains
Culture condition: 
37 °C, anaerobic
Information: 
<- ATCC <- NCTC<- M. Robertson (SR 5). Isolated from human, an acute case of gas gangrene. Used for sterility testing, media testing and pharmaceutical testing. Quality control strain.
Literature: 

Taxonomy/description (J Appl Bacteriol 1977, 43: 375-389). Metabolism of amino acids (J Gen Microbiol 1971, 67:47-56). Sterlity testing (British Pharmacopoeia Commission Test for sterility, British Pharmacopoeia Appendix XVI A, 2003; European Pharmacopoeia Commission Sterility, European Pharmacopoeia EP 2.6.1, 1997; U.S. Pharmacopeia General Chapters: <71> STERILITY TESTS, USP28-NF23, 2005; U.S. Pharmacopeia General Chapters:<62> Microbiological Examination of Nonsterile Products: Tests for Specified Microorganisms. Rockville, MD USP32-NF27, 2005). Media testing (ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions); British Pharmacopoeia Commission Tests for microbial contamination, British Pharmacopoeia Appendix XVI B, 2003; European Pharmacopoeia EP 2.6.13, 1997; Corry, JEL, Curtis, GDW and Baird, RM (Eds) Handbook of Culture Media for Food and Water Microbiology. Royal Society of Chemistry, In preparation. ICFMH-WPCM). Quality control strain (API Rapid ID 32 A. bioMerieux)

Supplied as: 
Freeze-dried
Risk group: 
2

Aspergillus brasiliensis

PTCC No.: 
5011
Other collection No.: 
ATCC 16404, CBS 733.88, DSM 1988, IFO 9455, IMI 149007,CIP 1431.83, WDCM 00053
Culture condition: 
24 °C
Information: 
<- ATCC <- S.M. Ringel, WLRI 034(120). Deposited as Aspergillus niger van Tieghem, anamorph. Isolated from blueberry, North Carolina, USA. Quality control strain. Used for assay of antimicrobial preservatives; media testing, sterility testing, fungicides testing, food testing and validation control. Applied in disinfectants and antiseptics testing. Reference strain for performance testing culture media listed by the ISO TC 34 SC 9 Joint Working Group 5 in the ISO 11133 and by the Working Party on Culture Media of the International Committee on Food Microbiology and Hygiene (ICFMH-WPCM)
Literature: 

Assay of antimicrobial preservatives (ASTM International Standard Test Method for Preservatives in Water-Containing Cosmetics. West Conshohocken, PA; British Pharmacopoeia Commission Efficacy of antimicrobial preservation. London, UK:British Pharmacopoeia Commission, British Pharmacopoeia Appendix XVI C, 2003; European Pharmacopoeia Commission Efficacy of antimicrobial preservation. Strasbourg, France:European Pharmacopoeia Commission, European Pharmacopoeia EP 5.1.3, 1997; U.S. Pharmacopeia General Chapters:<51> ANTIMICROBIAL EFFECTIVENESS TESTING. Rockville, MD:U.S. Pharmacopeia, USP USP28-NF23, 2005). Media testing (British Pharmacopoeia Commission Test for sterility. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI A, 2003, British Pharmacopoeia Commission Tests for microbial contamination. London, UK:British Pharmacopoeia Commission, British Pharmacopoeia Appendix XVI B, 2003; Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute, CLSI M22-A3; U.S. Pharmacopeia General Chapters:<51> ANTIMICROBIAL EFFECTIVENESS TESTING. Rockville, MD:U.S. Pharmacopeia, USP USP28-NF23, 2005; U.S. Pharmacopeia Dietary Supplement Chapters: <2021> MICROBIAL ENUMERATION TESTS-NUTRITIONAL AND DIETARY SUPPLEMENTS . Rockville, MD:U.S. Pharmacopeia, USP USP28-NF23, 2005; Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI, ISO ISO 11133-2:2003; Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution, British Standard DD CEN ISO/TS 11133:2003). Quality control strain (Applied Biosystems, MicroSeq Pharmaceutical Validation Panel; FF MicroPlate. Biolog; Applied Biosystems, MicroSeq PHL Panel). Sterility testing (U.S. Pharmacopeia General Chapters: <71> STERILITY TESTS. Rockville, MD:U.S. Pharmacopeia, USP USP28-NF23, 2005; Sterility test, Growth promotion test. Tokyo, Japan:Japanese Pharmacopoeia, JP JP14e.part I.54) Cleanrooms and associated controlled environments--Biocontamination control--Part 1: General principles and methods. Geneva (Switzerland):International Organization for Standardization/ANSI, ISO ISO 14698-1:2003). Optic lens antimicrobial preservative testing (Ophthalmic optics--Contact lens care products--Antimicrobial preservative efficacy testing and guidance on determining discard date. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 14730:2000). Testing fungicides (Chemical disinfectants and antiseptics -- Quantitative suspension test for the evaluation of fungicidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas -- Test method and requirements (phase 2, step 1). London, UK:British Standards Institution, British Standard BS EN 1650:1998;Chemical disinfectants and antiseptics - Preservation of test organisms used for the determination of bactericidal, sporicidal and fungicidal activity. London, UK:British Standards Institution, British Standard BS EN 12353:2006; Chemical disinfectants and antiseptics --- Quantitative non-porous surface test for the evaluation of bactericidal and/or fungicidal activity of chemical disinfectants used in food, industrial, domestic and institutional areas --- Test method and requirements without mechanical action (phase 2/step 2). London, UK:British Standards Institution, British Standard BS EN 13697:2001; Chemical disinfectants and antiseptics --- Quantitative suspension test for the evaluation of fungicidal activity of chemical disinfectants for instruments used in the medical area --- Test method and requirements (phase 2, step 1). London, UK:British Standards Institution, British Standard BS EN 13624:2003; Chemical disinfectants and antiseptics --- Quantitative suspension test for the evaluation of basic fungicidal or basic yeasticidal activity of chemical disinfectants and antiseptics --- Test method and requirements (phase 1). London, UK:British Standards Institution; British Standard BS EN 1275:2005; Chemical disinfectants and antiseptics --- Quantitative carrier test for the evaluation of fungicidal or yeasticidal activity for instruments used in the medical area --- Test method and requirements (phase 2, step 2). London, UK:British Standards Institution, British Standard BS EN 14562:2006; Chemical disinfectants and antiseptics --- Quantitative suspension test for the evaluation of fungicidal or yeasticidal activity of chemical disinfectants and antiseptics used in the veterinary area --- Test method and requirements (phase 2, step 1). London, UK:British Standards Institution, British Standard BS EN 1657:2005; Chemical disinfectants and antiseptics --- Application of European Standards for chemical disinfectants and antiseptics. London, UK:British Standards Institution, British Standard BS EN 14885:2006). Transformation host (Curr Genet 1994, 25: 519-523. PubMed: 8082203; Gene 1993, 124: 239-244. PubMed: 8444347). Validation control (Cleanrooms and associated controlled environments --- Biocontamination control --- Part 1: General principles and methods. London, UK:British Standards Institution, British Standard BS EN ISO 14698:2003; Clean room biocontamination control (Cleanrooms and associated controlled environments--Biocontamination control--Part 1: General principles and methods. Geneva (Switzerland):International Organization for Standardization/ANSI, ISO ISO 14698-1:2003))

Supplied as: 
Freeze-dried
Risk group: 
1

Citrobacter freundii

PTCC No: 
1772
Other collection No. :: 
ATCC 43864, DSM 24397, CIP 103547, LMG 21265, CCUG 53829, WDCM 00006
Category: 
Quality control strains
Culture condition: 
37 °C, Aerobic
Information: 
<- ATCC <- API System/ bioMerieux, Strain LRA 117.03.76. Human clinical isolate, Montpellier; France. Used for Media testing, Quality control strain and Quality control strain for API products. Contains qnrB-like pseudogene (plasmid-mediated quinolone resistance gene)
Literature: 

Media testing (Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003, Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003, ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions), Corry, JEL, Curtis, GDW and Baird, RM (Eds) Handbook of Culture Media for Food and Water Microbiology. Royal Society of Chemistry, In preparation. ICFMH-WPCM. qnrB-like pseudogene (Antimicrob Agents Chemother  2013, 57 (6): 2863-2866)

Supplied as: 
Freeze-dried
Risk group: 
2

Lactococcus lactis subsp.lactis

PTCC No: 
1403
Other collection No. :: 
CIP 70.56, ATCC 19435, NCTC 6681, DSM 20481, NCIB 6681, WDCM 00016
Category: 
Quality control strains
Culture condition: 
30°C, 5% CO2
Information: 
<- CIP <- P. Cayeux, Inst. Pasteur, Paris, France: strain N30 <- Shattock P.M.F., Reading, UK: strain lactis OJ. Synonym(s): Streptococcus lactis (Lister 1873) Löhnis 1909. Lactobacillus xylosus Kitahara 1938. Streptococcus lactis subsp. diacetilactis (ex Matuszewski et al. 1936) Garvie and Farrow 1982. Streptococcus lactis subsp. lactis (Lister 1873) Garvie and Farrow 1982. Lactococcus lactis subsp. lactis (Lister 1873) Schleifer et al. 1986. Type strain, Serological group N. Used for media testing.
Literature: 

Taxonomy ( Int. J. Syst. Bacteriol. 1980, 30: 368; Int. J. Syst. Bacteriol., 1986 36: 354 ; Int. J. Syst. Bacteriol., 1999, 49, 163 ; System. Appl. Microbiol., 1985, 6, 183 - 195), media testing (International Organization for Standardization/ANSI;ISO ISO 11133-2:2003; British Standard DD CEN ISO/TS 11133:2003, ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions, Corry, JEL, Curtis, GDW and Baird, RM (Eds) Handbook of Culture Media for Food and Water Microbiology. Royal Society of Chemistry, In preparation. ICFMH-WPCM), Antimicrobial susceptibilities (J Clin Microbiol 1996, 34: 1296-1298, PubMed: 8727924

Supplied as: 
Freeze-dried
Risk group: 
1

Citrobacter freundii

PTCC No: 
1600
Other collection No. :: 
DSM 30039, NCIMB 11490, ATCC 8090, NCTC 9750, IFO 12681, NBRC 12681
Category: 
Quality control strains
Reference strains
Culture condition: 
37 °C
Information: 
<- ATCC <- C.H. Werkman. Type strain. Produces restriction endonuclease CfrAI. Quality control strain
Literature: 

Taxonomy (Int J Syst Bacteriol 1980, 30 : 225-420. Int J Syst Bacteriol1999, 49 : 1433-1438). Quality Control strain (Water microbiology. Method 14: Salmonellae. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.14-1995. Medizin, D. I. f. N. N., (1997). DIN 58959-7)

Supplied as: 
Freeze-dried
Risk group: 
2

Enterococcus faecalis

PTCC No: 
1774
Other collection No. :: 
ATCC 19433, DSM 20478, CCUG 19916, JCM 7826, LMG 7937, NCIMB 775,NCDO 581, NCTC 775, CIP 103015, WDCM 00009
Category: 
Quality control strains
Reference strains
Culture condition: 
37 °C, aerobic
Information: 
<- ATCC <- NCTC <- NIRD. Serological group D, Type strain. Quality control strain used for media testing and water testing. Quality control strain for Difco products. Reference strain.
Literature: 

Taxonomy (Int J Syst Bacteriol 1984, 34: 31-34). Media testing (Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003., ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions), Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermenation using Lauryl Tryptose Broth (LTB) and EC Medium. Washington, DC:Environmental Protection Agency; EPA EPA Method 1680, 2005). Water testing (Escherichia coli in Water by Membrane Filtration using membrane-Thermotolerant Escherichia coli Agar (mTEC). Washington, DC:Environmental Protection Agency;EPA EPA Method 1103.1, 2005, Enterococci in Water by Membrane Filtration Using membrane-Enterococcus-Esculin Iron Agar. Washington, DC:Environmental Protection Agency;EPA EPA Method 1106.1, 2005, Enterococci in Water by Membrane Filtration Using membrane-Enterococcus Indoxyl-B-D-Glucoside Agar. Washington, DC:Environmental Protection Agency;EPA EPA Method 1600, 2005, Escherichia coli in Water by Membrane Filtration Using Modified membrane-Thermotolerant Escherichia coli Agar. Washington, DC:Environmental Protection Agency;EPA EPA Method 1603, 2005)

Supplied as: 
Freeze-dried
Risk group: 
2

Klebsiella aerogenes

PTCC No: 
1221
Other collection No. :: 
NCTC 10006, ATCC 13048, DSM 30053, CDC 813-60, WDCM 00175
Category: 
Quality control strains
Reference strains
Culture condition: 
30°C, Aerobic
Information: 
<- NCTC <- Edwards P. R. Isolated from sputum, South Carolina Dept. of Health and Environmental Control. Previous Catalogue Name: Enterobacter aerogenes; Type strain. Used for Assay of antimicrobial preservatives, bacterial resistance testing adhesives, testing bacterial resistance of paints, and testing slimicide. Quality control strain and quality control strain for BBL, bioMerieux Vitek, and Difco products. Used for water testing and media testing
Literature: 

Taxonomy (Int J Syst Bacteriol 1980, 30: 225-420; Int J Syst Evol Microbiol 2002, 52: 531-47; J Clin Microbiol 2002, 40:2779-85 ), quality control strain (DIN 58959-7, 1997), degradation of HCH (Arch Microbiol 115: 285-292). Testing slimicide (Standard Test Method for Efficacy of Slimicides for the Paper Industry-Bacterial and Fungal Slime. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1839-07). Paint resistance (ASTM International Standard Test Method for Resistance of Emulsion Paints in the Container to Attack by Microorganisms. West Conshohocken, PA:ASTM International;ASTM Standard Test Method D 2574-06). Water testing (Water microbiology. Method 4: Coliforms--Estimation of most probable number (MPN). Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.4-1995. Water microbiology. Method 5: Coliforms--Membrane filtration method. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.5-1995. Water microbiology. Method 6: Thermotolerant coliforms and Escherichia coli--Estimation of most probable number (MPN). Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.6-1995. Water microbiology. Method 7: Thermotolerant coliforms and Escherichia coli -- Membrane filtration method. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.7-1995. Water microbiology. Method 11: Pseudomonads -- Membrane filtration method. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.11-1995. Water microbiology. Method 18: Aeromonas by membrane filtration including selected speciation. Sydney, NSW, Australia:Standards Australia;Standards Australia AS/NZS 4276.18:2001). Sanitizer Test (Sanitizer Test or Inanimate Surfaces. Washington, DC:Environmental Protection Agency;EPA EPA DIS/TSS-10). Enumeration of Coliforms (Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermenation using Lauryl Tryptose Broth (LTB) and EC Medium. Washington, DC:Environmental Protection Agency;EPA EPA Method 1680, 2005. Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermenatation using A-1 medium. Washington, DC:Environmental Protection Agency;EPA EPA Method 1681, 2005). Media testing (ISO 11133, Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media)

Supplied as: 
Freeze-dried
Risk group: 
2

Escherichia coli

PTCC No: 
1399
Other collection No. :: 
ATCC 25922, DSM 1103, NCIB 12210, CCUG 17620, WDCM 00013
Category: 
Quality control strains
Reference strains
Culture condition: 
37°C, Aerobic
Information: 
<- ATCC <- FDA, strain Seattle 1946 <- F. Schoenknecht. Clinical isolate. Control strain for susceptibility testing of bacteria to antibiotics. Sensitivity testing to cephaloglycin dihydrate, cephalexin monohydrate, cephaloridine and sodium cephalothin; standardized assays, including antibiotic disc susceptibility testing and MIC of ampicillin, bacitracin, cephalothin, carbenicillin, chloramphenicol, colistin (colimycin), gentamicin, kanamycin, neomycin, nitrofurantoin, polymyxin B, streptomycin, tetracycline, tobramycin and trimethoprim-sulfamethoxazole. Recommended as international reference standard strain for antibiotic disc susceptibility testing. Modified Kirby-Bauer susceptibility testing of chloramphenicol, colistin, fosfomycin, gentamicin, kanamycin, neomycin, nalidixic acid and tetracycline discs. Quality control strain for media testing. Used for evaluation of Mueller-Hinton agar, food testing. Quality control strain for Abbott, API, Autobac, BBL, bioMerieux Vitek, Biosynth, Difco, IDS, Micro-Media, MicroScan, Roche Diagnostics, and Sensititre products. Quality control strain for EUCAST. Bruker Quality Control Strain (MALDI-TOF).
Literature: 

Control Culture (Identification of Foodborne Bacterial Pathogens by Gene Probes: Enterotoxigenic Escherichia coli: Heat-Stable Enterotoxin (Human), Heat-Stable Enterotoxin (Porcine), and Heat-Labile Enterotoxin. Washington, DC:US Food & Drug Administration, Bacteriological Analytical Manual Online Chapt. 24, 2001; Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermenatation using A-1 medium. Washington, DC:Environmental Protection Agency, EPA EPA Method 1681, 2005; Salmonella in Sewage Sludge (Biosolids) by Modified Semisolid Rappaport-Vassiliadis (MSRV) Medium. Washington, DC:Environmental Protection Agency, EPA EPA Method 1682, 2005). Control strain (Methodology for the Serum Bactericidal test: Approved Guideline. Wayne, PA. Clinical and Laboratory Standards Institute, CLSI M21-A; Methods for Determining Bactericidal Activity of Antimicrobial Agents; Approved Guideline. Wayne, PA. Clinical and Laboratory Standards Institute, CLSI M26-A; Procedures for the Recovery and Identification of Parasites from the Intestinal Tract: Approved Guideline - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute, CLSI M28-A2). Detection of coliform bacteria (Enumeration of Escherichia coli and the Coliform Bacteria: LST-MUG Method for Detecting E.coli in chilled or Frozen Foods Exclusive of Bivalve Mollucan Sellfish. Washington, DC:US Food & Drug Administration, Bacteriological Analytical Manual Online Chapt. 4.II.3, 2002;Salmonella in Foods. Gaithersburg, MD:AOAC International, AOAC "Official Methods of Analysis of the AOAC International" 991.38;Microbiology of food and animal feeding stuffs-- Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species)--Part3: Detection and MPN technique for low numbers. Geneva (Switzerland):International Organization for Standardization/ANSI, ISO ISO 6888-3:2003; Microbiology of food and animal feeding stuffs --- Horizontal method for the determination of low numbers of presumptive Bacillus cereus --- Most probable number technique and detection method. London, UK:British Standards Institution, British Standard BS EN ISO 21871:2006). Evaluation of Mueller-Hinton agar(Protocols for Evaluating Dehydrated Mueller-Hinton Agar; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute, CLSI M6-A2; US General Services Administration Mueller Hinton Medium, dehydrated. Washington, DC:US General Services Administration, Commercial Item Description A-A-50904A, 1986). Media testing (Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute, CLSI M22-A3; Food microbiology. Method 12.3: Microbiology of food and animal feeding stuffs -- Horizontal method for the enumeration of coagulase-positive staphylococci (Staphyloccus aureus and other species)--Detection and MPN technique for low numbers. Sydney, NSW, Australia:Standards Australia, Standards Australia AS 5013.12.3:2004;Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI, ISO ISO 11133-2:2003; Medical microbiology-- Culture media -- Part 3: Dip slides for microbiological urine analysis. Berlin, Germany:Deutsches Institut fur Normung, DIN DIN 58942-3: 2003; Microbiology of food and animal feeding stuffs --- Horizontal method for the detection and enumeration of Listeria monocytogenes --- Part 1: Detection method, Annex B [BS 5763-18:1997]. London, UK:British Standards Institution, British Standard BS EN ISO 11290-1:1997; Microbiology of food and animal feeding stuffs --- Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli -- Part 3: Most probable number techniques using 5-bromo-4-chloro-3-indolyl-beta-D-glucuronide. London, UK:British Standards Institution, British Standard DD ISO/TS 16649-3:2005; Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution, British Standard DD CEN ISO/TS 11133:2003). Quality control strain (Quality Control of Microbiological Transport Systems; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute, CLSI M40-A). Susceptibility disc testing ( Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - 9th Edition. Wayne, PA. Clinical and Laboratory Standards Institute, CLSI M2-A9; Methods for Antimicrobial Disk Susceptibility Testing of Bacteria Isolated from Aquatic Animals. Wayne, PA. Clinical and Laboratory Standards Institute, CLSI M42-A; Methods for Antimicrobial Dilution and Disk susceptibility Testing of Infrequently Isolated or Fastidious Bacteria: Proposed Guideline. Wayne, PA. Clinical and Laboratory Standards Institute, CLSI M45-A; Methods for Broth Dilution Susceptibility Testing of Bacteria Isolated from Aquatic Animals. Wayne, PA. Clinical and Laboratory Standards Institute, CLSI M49-A). Susceptibility disc testing chloramphenico, colistin colimycin, gentamicins, kanamycin, nalidixic acid, neomycin, tetracycline (Antimicrob Agents Chemother 1973, 3: 418-424, PubMed: 4790600). Susceptibility testing (Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard - 7th Edition. Wayne, PA. Clinical and Laboratory Standards Institute, CLSI M7-A8; Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria. Wayne, PA. Clinical and Laboratory Standards Institute, CLSI M11-A7; Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute, CLSI M31-A3; Performance Standards for Antimicrobial Susceptibility Testing; Sixteenth Informational Supplement. Wayne, PA. Clinical and Laboratory Standards Institute, CLSI M100-S20, 2007; Evaluation of Lots of Dehydrated Mueller-Hinton Broth for Antimicrobial Susceptibility testing; Proposed Guideline. Wayne, PA. Clinical and Laboratory Standards Institute, CLSI M32-P; Medical microbiology; methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents: agar diffusion test. Berlin, Germany: Deutsches Institut fur Normung, DIN 58940-3: 1989; Medical microbiology -- Methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents-- Part 7: Determination of the minimum bactericidal concentration (MBC) with the aid of microdilution. Berlin, Germany:Deutsches Institut fur Normung, DIN DIN 58940-7: 1994; Part 81: Microdilution; Special requirements for testing of non-fastidious bacteria. Berlin, Germany: Deutsches Institut fur Normung, DIN 58940-81: 2002;Clinical laboratory testing and in vitro diagnostic test systems --- Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices --- Part 1: Reference method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases. London, UK:British Standards Institution, British Standard BS EN ISO 20776-1:2006). Control strain (Aeromonas in Finished Water by Membrane Filtration using Ampicillin-Dextrin Agar with Vancomycin (ADA-V). Washington, DC:Environmental Protection Agency ,EPA EPA Method 1605, 2001).

Supplied as: 
Freeze-dried
Risk group: 
2

Enterococcus faecalis

PTCC No: 
1778
Other collection No. :: 
ATCC 29212, DSM 2570, CIP 103214, LMG 8222, WDCM 00087
Category: 
Quality control strains
Reference strains
Culture condition: 
37°C, Aerobic
Information: 
<- ATCC <- Micro-media System Inc. <- Kaiser-Permanente, strain Portland. Isolated from urine. Quality control strain. Used for food testing, evaluation of Mueller-Hinton agar, media testing, susceptibility disc testing and dilution antimicrobial susceptibility testing. Quality control strain for API, bioMerieux VITEK, IDS, Micro-Media, MicroScan, and Sensititre products. Quality control strain for EUCAST (test for minimum inhibitory concentration of antibiotics).
Literature: 

Control strain (Methodology for the Serum Bactericidal test: Approved Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M21-A, Methods for Determining Bactericidal Activity of Antimicrobial Agents; Approved Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M26-A). Media testing (Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3, Water microbiology. Method2: Culture media, diluents and reagents. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.2-1995, Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003, Medical microbiology-- Culture media -- Part 3: Dip slides for microbiological urine analysis. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58942-3: 2003, 2003, Microbiology of food and animal feeding stuffs --- Horizontal method for the detection and enumeration of Listeria monocytogenes --- Part 1: Detection method, Annex B [BS 5763-18:1997]. London, UK:British Standards Institution;British Standard BS EN ISO 11290-1:1997, Microbiology of food and animal feeding stuffs --- Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli -- Part 3: Most probable number techniques using 5-bromo-4-chloro-3-indolyl-beta-D-glucuronide. London, UK:British Standards Institution;British Standard DD ISO/TS 16649-3:2005, Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003), Quality control of transport medium (Quality Control of Microbiological Transport Systems; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M40-A). Susceptibility disc testing (Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - 9th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M2-A9, Methods for Antimicrobial Disk Susceptibility Testing of Bacteria Isolated from Aquatic Animals. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M42-A, Methods for Broth Dilution Susceptibility Testing of Bacteria Isolated from Aquatic Animals. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M49-A). Susceptibility testing (Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard - 7th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M7-A8, Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M31-A3, Performance Standards for Antimicrobial Susceptibility Testing; Sixteenth Informational Supplement. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M100-S20, 2007, Evaluation of Lots of Dehydrated Mueller-Hinton Broth for Antimicrobial Susceptibility testing; Proposed Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M32-P, Medical microbiology; methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents: agar diffusion test. Berlin, Germany: Deutsches Institut fur Normung; DIN 58940-3: 1989, Medical microbiology -- Methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents-- Part 7: Determination of the minimum bactericidal concentration (MBC) with the aid of microdilution. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58940-7: 1994, 1994, Medical microbiology -- Susceptibility testing of microbial pathogens to antimicrobial agents -- Part 81: Microdilution; Special requirements for testing of non-fastidious bacteria. Berlin, Germany: Deutsches Institut fur Normung; DIN 58940-81: 2002, Clinical laboratory testing and in vitro diagnostic test systems --- Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices --- Part 1: Reference method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases. London, UK:British Standards Institution;British Standard BS EN ISO 20776-1:2006)

Supplied as: 
Freeze-dried
Risk group: 
2

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