Clostridium perfringens
Production of phospholipase C (alpha-toxin), haemolysins and lethal toxins (J Gen Microbiol 1976, 96:137-144). Production of lecithinase C (J Appl Bacteriol 1959, 22:1). Quality control strain for media testing (British Pharmacopoeia Commission Tests for microbial contamination. London, UK:British Pharmacopoeia Commission, British Pharmacopoeia Appendix XVI B, 2003; European Pharmacopoeia Commission Microbial contamination of products not required to comply with the test for sterility (tests for specified micro-organisms). Nutritive and selective properties of the media and validity of the test for specified micro-organisms. Strasbourg, France:European Pharmacopoeia Commission, European Pharmacopoeia EP 2.6.13, 1997; Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3; Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI, ISO ISO 11133-2:2003; Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution, British Standard DD CEN ISO/TS 11133:2003). Quality control strain (US General Services Administration Test kit, anaerobe identification. Washington, DC:US General Services Administration, Commercial Item Description A-A-51238, 1985). Reference strain (Water microbiology. Method 17.1: Spores of sulfite-reducing anaerobes (clostridia) including Clostridium perfringens --Membrane filtration method. Sydney, NSW, Australia:Standards Australia, Standards Australia AS/NZS 4276.17.1:2000; Water microbiology. Method 17.2: Spores of sulfite-reducing anaerobes (clostridia) including Clostridium perfringens -- Estimation of most probable number (MPN) using the multiple tube dilution technique.. Sydney, NSW, Australia:Standards Australia, Standards Australia AS/NZS 4276.17.2:2000; Water microbiology. Method 18: Aeromonas by membrane filtration including selected speciation. Sydney, NSW, Australia:Standards Australia, Standards Australia AS/NZS 4276.18:2001). Quality control strain for anaerobe identification ( US General Services Administration Test kit, anaerobe identification. Washington, DC:US General Services Administration; Commercial Item Description A-A-51238,1985). Contol strain for antimicrobic susceptibility testing of anaerobic bacteria (Antimicrob Agents Chemother 1979, 16:495-502).