Peptone 5.0 g
Meat extract 3.0 g
NaCl 5.0 g
Agar 15.0 g
Distilled water 1.0 L
Adjust pH to 7.0.
For sporulation of Bacillus strains the addition of 10.0 mg MnCl2.2H2O, 100 mg CaCl2.2H2O, and 500 mg MgSo4.7H2o is recommended.
