MRS BROTH MEDIUM

Casein peptone, tryptic digest 10.0 g

Meat extract 10.0 g

Yeast extract 5.0 g

Glucose 20.0 g

Tween 80 1.0 g

K2HPO4 2.0 g

Sodium acetate 5.0 g

Diammonium citrate 2.0 g

MgSO4.7H2O 0.2 g

MnSO4.H2O 0.05 g

Distilled water 1.0 L

Adjust pH to 6.2 6.5

Lactobacilli MRS Broth (Difco 288130)