Reference strains

Penicillium pinophilum

PTCC No.: 
5168
Other collection No.: 
CBS 170.60, ATCC 9644, NRRL A-5245, WDCM 00194
Culture condition: 
24 °C
Information: 
<- CBS <- ATCC <- WH Weston <- K.L. Jensen SN 41. Isolated from radio set, Papua New Guinea. Previous name: Penicillium funiculosum. Used for fungus resistance testing adhesives, fungus resistance testing aircraft transmissions, fungus resistance testing cork, fungus resistance testing cork, fungus resistance testing electrical insulation, fungus resistance testing packing materials, fungus resistance testing paper and paperboard, fungus resistance testing plastics, fungus resistance testing varnish, fungus resistance testing wax, testing fungicides on lumber. Production of dextranase and poly-beta-hydroxybutyrate depolymerase. Degrades plastics, poly-beta-hydroxybutyrate poly 3-hydroxybutyrate, polycaprolactone.
Literature: 

Fungus resistance testing adhesives (ASTM International Standard Test Methods for Resistance of Adhesive Preparations in Container to Attack by Bacteria, Yeast, and Fungi. West Conshohocken, PA:ASTM International; ASTM Standard Test Method D 4783-01e1; ASTM International Standard Test Methods for Ability of Adhesive Films to Support or Resist the Growth of Fungi. West Conshohocken, PA:ASTM International; ASTM Standard Test Method D 4300-01, Adhesives for load-bearing timber structures - Casein adhesives - Classification and performance requirements, Annex B. London, UK:British Standards Institution; British Standard BS EN 12436:2002). Degradation of plastics (Int Biodeterior Bull 1972, 8: 3-7; Dev Ind Microbiol 1973,14: 258-263). Degradation of poly-beta-hydroxybutyrate poly3-hydroxybutyrate, polycaprolactone (J Ferment Bioeng 1995, 80: 265-269). Fungus resistance testing electrical insulation (U.S. Department of Defense Insulation, electrical, synthetic-resin composition, nonrigid. Washington, DC:US Department of Defense;Military Specification MIL-I-631D, 1961). Fungus resistance testing paper and paperboard (Standard Test Method for Efficacy of Fungal Control Agents as Preservatives for Aqueous-Based Products Used in the Paper Industry. West Conshohocken, PA:ASTM International; ASTM Standard Test Method E 0875-00 (Reapproved 2005). Fungus resistance testing plastics (Plastics--Assessment of the effectiveness of fungistatic compounds in plastics formulations. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 16869:2001). Fungus resistance testing wax (U.S. Department of Defense Wax, impregnating, waterproofing, for laminated paper tubes for small arms ammunition. Washington, DC:US Department of Defense; Military Specification MIL-W-10885B, 1956). Production of dextranase (J Biochem 1971, 69: 1113-1121). Production ofpoly-beta-hydroxybutyrate depolymerase (Arch Biochem Biophys 1991, 290: 497-502, PubMed: 1929416)

Supplied as: 
Freeze-dried
Risk group: 
1

Lactobacillus acidophilus

PTCC No: 
1643
Other collection No. :: 
DSM 20079, ATCC 4356, NCDO 1748, NCIB 8690, WDCM 00098
Category: 
Reference strains
Culture condition: 
37 °C, anaerobic
Information: 
<- DSMZ <-<- P.A. Hansen, L917 <- W.L. Kulp, strain Scav.Isolated from human. Type strain. Produces hydrogen peroxide
Literature: 

Taxonomy (Int. J. Syst. Bacteriol. 1980, 30:53-68; Int J Syst Bacteriol 1980, 30: 225-420). Hydrogen peroxide production (Zheng HY, et al. Effects of H2O2-producing lactobacilli on Neisseria gonorrhoeae growth and catalase activity. J. Infect. Dis. 1994, 170: 1209-1215). Reference strain (SO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions)

Supplied as: 
Freeze-dried
Risk group: 
1

Enterococcus faecalis

PTCC No: 
1774
Other collection No. :: 
ATCC 19433, DSM 20478, CCUG 19916, JCM 7826, LMG 7937, NCIMB 775,NCDO 581, NCTC 775, CIP 103015, WDCM 00009
Category: 
Quality control strains
Reference strains
Culture condition: 
37 °C, aerobic
Information: 
<- ATCC <- NCTC <- NIRD. Serological group D, Type strain. Quality control strain used for media testing and water testing. Quality control strain for Difco products. Reference strain.
Literature: 

Taxonomy (Int J Syst Bacteriol 1984, 34: 31-34). Media testing (Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003., ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions), Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermenation using Lauryl Tryptose Broth (LTB) and EC Medium. Washington, DC:Environmental Protection Agency; EPA EPA Method 1680, 2005). Water testing (Escherichia coli in Water by Membrane Filtration using membrane-Thermotolerant Escherichia coli Agar (mTEC). Washington, DC:Environmental Protection Agency;EPA EPA Method 1103.1, 2005, Enterococci in Water by Membrane Filtration Using membrane-Enterococcus-Esculin Iron Agar. Washington, DC:Environmental Protection Agency;EPA EPA Method 1106.1, 2005, Enterococci in Water by Membrane Filtration Using membrane-Enterococcus Indoxyl-B-D-Glucoside Agar. Washington, DC:Environmental Protection Agency;EPA EPA Method 1600, 2005, Escherichia coli in Water by Membrane Filtration Using Modified membrane-Thermotolerant Escherichia coli Agar. Washington, DC:Environmental Protection Agency;EPA EPA Method 1603, 2005)

Supplied as: 
Freeze-dried
Risk group: 
2

Bacillus pumilus

PTCC No: 
1319
Other collection No. :: 
NCIMB 8982, NCTC 8241, ATCC 14884, DSM 361, CIP 3.83, LMG 18517,
Category: 
Reference strains
Culture condition: 
30 °C
Information: 
<- NCIMB <- NCTC<- Humphrey J.H., strain Mill Hill. Used for assay of neomycin and Chlorotetracycline, assay of erythromycin, assay of framycetin, assay of gentamicin, assay of lymecycline, assay of tetracycline and oxytetracycline. Used for sterilization control and pharmaceutical and personal care.
Literature: 

Assay of chlortetracycline aureomycin, lymecycline, oxytetracycline terramycin and tetracycline (British Pharmacopoeia Commission Biological assay of antibiotics. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XIV A, 2003). Assay of erythromycin, framycetin, gentamicins gentamicin, neomycin (British Pharmacopoeia Commission Biological assay of antibiotics. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XIV A, 2003; European Pharmacopoeia Commission Microbiological assay of antibiotics. Strasbourg, France:European Pharmacopoeia Commission; European Pharmacopoeia EP 2.7.2, 1997). Sterilization control (Biological systems for sterilizers and sterilization processes --- Part 4: Particular systems for use in irradiation sterilizers. London, UK:British Standards Institution;British Standard BS EN 866-4:2000).

Supplied as: 
Freeze-dried
Risk group: 
1

Klebsiella aerogenes

PTCC No: 
1221
Other collection No. :: 
NCTC 10006, ATCC 13048, DSM 30053, CDC 813-60, WDCM 00175
Category: 
Quality control strains
Reference strains
Culture condition: 
30°C, Aerobic
Information: 
<- NCTC <- Edwards P. R. Isolated from sputum, South Carolina Dept. of Health and Environmental Control. Previous Catalogue Name: Enterobacter aerogenes; Type strain. Used for Assay of antimicrobial preservatives, bacterial resistance testing adhesives, testing bacterial resistance of paints, and testing slimicide. Quality control strain and quality control strain for BBL, bioMerieux Vitek, and Difco products. Used for water testing and media testing
Literature: 

Taxonomy (Int J Syst Bacteriol 1980, 30: 225-420; Int J Syst Evol Microbiol 2002, 52: 531-47; J Clin Microbiol 2002, 40:2779-85 ), quality control strain (DIN 58959-7, 1997), degradation of HCH (Arch Microbiol 115: 285-292). Testing slimicide (Standard Test Method for Efficacy of Slimicides for the Paper Industry-Bacterial and Fungal Slime. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1839-07). Paint resistance (ASTM International Standard Test Method for Resistance of Emulsion Paints in the Container to Attack by Microorganisms. West Conshohocken, PA:ASTM International;ASTM Standard Test Method D 2574-06). Water testing (Water microbiology. Method 4: Coliforms--Estimation of most probable number (MPN). Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.4-1995. Water microbiology. Method 5: Coliforms--Membrane filtration method. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.5-1995. Water microbiology. Method 6: Thermotolerant coliforms and Escherichia coli--Estimation of most probable number (MPN). Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.6-1995. Water microbiology. Method 7: Thermotolerant coliforms and Escherichia coli -- Membrane filtration method. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.7-1995. Water microbiology. Method 11: Pseudomonads -- Membrane filtration method. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.11-1995. Water microbiology. Method 18: Aeromonas by membrane filtration including selected speciation. Sydney, NSW, Australia:Standards Australia;Standards Australia AS/NZS 4276.18:2001). Sanitizer Test (Sanitizer Test or Inanimate Surfaces. Washington, DC:Environmental Protection Agency;EPA EPA DIS/TSS-10). Enumeration of Coliforms (Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermenation using Lauryl Tryptose Broth (LTB) and EC Medium. Washington, DC:Environmental Protection Agency;EPA EPA Method 1680, 2005. Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermenatation using A-1 medium. Washington, DC:Environmental Protection Agency;EPA EPA Method 1681, 2005). Media testing (ISO 11133, Microbiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media)

Supplied as: 
Freeze-dried
Risk group: 
2

Escherichia coli

PTCC No: 
1399
Other collection No. :: 
ATCC 25922, DSM 1103, NCIB 12210, CCUG 17620, WDCM 00013
Category: 
Quality control strains
Reference strains
Culture condition: 
37°C, Aerobic
Information: 
<- ATCC <- FDA, strain Seattle 1946 <- F. Schoenknecht. Clinical isolate. Control strain for susceptibility testing of bacteria to antibiotics. Sensitivity testing to cephaloglycin dihydrate, cephalexin monohydrate, cephaloridine and sodium cephalothin; standardized assays, including antibiotic disc susceptibility testing and MIC of ampicillin, bacitracin, cephalothin, carbenicillin, chloramphenicol, colistin (colimycin), gentamicin, kanamycin, neomycin, nitrofurantoin, polymyxin B, streptomycin, tetracycline, tobramycin and trimethoprim-sulfamethoxazole. Recommended as international reference standard strain for antibiotic disc susceptibility testing. Modified Kirby-Bauer susceptibility testing of chloramphenicol, colistin, fosfomycin, gentamicin, kanamycin, neomycin, nalidixic acid and tetracycline discs. Quality control strain for media testing. Used for evaluation of Mueller-Hinton agar, food testing. Quality control strain for Abbott, API, Autobac, BBL, bioMerieux Vitek, Biosynth, Difco, IDS, Micro-Media, MicroScan, Roche Diagnostics, and Sensititre products. Quality control strain for EUCAST. Bruker Quality Control Strain (MALDI-TOF).
Literature: 

Control Culture (Identification of Foodborne Bacterial Pathogens by Gene Probes: Enterotoxigenic Escherichia coli: Heat-Stable Enterotoxin (Human), Heat-Stable Enterotoxin (Porcine), and Heat-Labile Enterotoxin. Washington, DC:US Food & Drug Administration, Bacteriological Analytical Manual Online Chapt. 24, 2001; Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermenatation using A-1 medium. Washington, DC:Environmental Protection Agency, EPA EPA Method 1681, 2005; Salmonella in Sewage Sludge (Biosolids) by Modified Semisolid Rappaport-Vassiliadis (MSRV) Medium. Washington, DC:Environmental Protection Agency, EPA EPA Method 1682, 2005). Control strain (Methodology for the Serum Bactericidal test: Approved Guideline. Wayne, PA. Clinical and Laboratory Standards Institute, CLSI M21-A; Methods for Determining Bactericidal Activity of Antimicrobial Agents; Approved Guideline. Wayne, PA. Clinical and Laboratory Standards Institute, CLSI M26-A; Procedures for the Recovery and Identification of Parasites from the Intestinal Tract: Approved Guideline - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute, CLSI M28-A2). Detection of coliform bacteria (Enumeration of Escherichia coli and the Coliform Bacteria: LST-MUG Method for Detecting E.coli in chilled or Frozen Foods Exclusive of Bivalve Mollucan Sellfish. Washington, DC:US Food & Drug Administration, Bacteriological Analytical Manual Online Chapt. 4.II.3, 2002;Salmonella in Foods. Gaithersburg, MD:AOAC International, AOAC "Official Methods of Analysis of the AOAC International" 991.38;Microbiology of food and animal feeding stuffs-- Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species)--Part3: Detection and MPN technique for low numbers. Geneva (Switzerland):International Organization for Standardization/ANSI, ISO ISO 6888-3:2003; Microbiology of food and animal feeding stuffs --- Horizontal method for the determination of low numbers of presumptive Bacillus cereus --- Most probable number technique and detection method. London, UK:British Standards Institution, British Standard BS EN ISO 21871:2006). Evaluation of Mueller-Hinton agar(Protocols for Evaluating Dehydrated Mueller-Hinton Agar; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute, CLSI M6-A2; US General Services Administration Mueller Hinton Medium, dehydrated. Washington, DC:US General Services Administration, Commercial Item Description A-A-50904A, 1986). Media testing (Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute, CLSI M22-A3; Food microbiology. Method 12.3: Microbiology of food and animal feeding stuffs -- Horizontal method for the enumeration of coagulase-positive staphylococci (Staphyloccus aureus and other species)--Detection and MPN technique for low numbers. Sydney, NSW, Australia:Standards Australia, Standards Australia AS 5013.12.3:2004;Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI, ISO ISO 11133-2:2003; Medical microbiology-- Culture media -- Part 3: Dip slides for microbiological urine analysis. Berlin, Germany:Deutsches Institut fur Normung, DIN DIN 58942-3: 2003; Microbiology of food and animal feeding stuffs --- Horizontal method for the detection and enumeration of Listeria monocytogenes --- Part 1: Detection method, Annex B [BS 5763-18:1997]. London, UK:British Standards Institution, British Standard BS EN ISO 11290-1:1997; Microbiology of food and animal feeding stuffs --- Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli -- Part 3: Most probable number techniques using 5-bromo-4-chloro-3-indolyl-beta-D-glucuronide. London, UK:British Standards Institution, British Standard DD ISO/TS 16649-3:2005; Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution, British Standard DD CEN ISO/TS 11133:2003). Quality control strain (Quality Control of Microbiological Transport Systems; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute, CLSI M40-A). Susceptibility disc testing ( Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - 9th Edition. Wayne, PA. Clinical and Laboratory Standards Institute, CLSI M2-A9; Methods for Antimicrobial Disk Susceptibility Testing of Bacteria Isolated from Aquatic Animals. Wayne, PA. Clinical and Laboratory Standards Institute, CLSI M42-A; Methods for Antimicrobial Dilution and Disk susceptibility Testing of Infrequently Isolated or Fastidious Bacteria: Proposed Guideline. Wayne, PA. Clinical and Laboratory Standards Institute, CLSI M45-A; Methods for Broth Dilution Susceptibility Testing of Bacteria Isolated from Aquatic Animals. Wayne, PA. Clinical and Laboratory Standards Institute, CLSI M49-A). Susceptibility disc testing chloramphenico, colistin colimycin, gentamicins, kanamycin, nalidixic acid, neomycin, tetracycline (Antimicrob Agents Chemother 1973, 3: 418-424, PubMed: 4790600). Susceptibility testing (Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard - 7th Edition. Wayne, PA. Clinical and Laboratory Standards Institute, CLSI M7-A8; Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria. Wayne, PA. Clinical and Laboratory Standards Institute, CLSI M11-A7; Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute, CLSI M31-A3; Performance Standards for Antimicrobial Susceptibility Testing; Sixteenth Informational Supplement. Wayne, PA. Clinical and Laboratory Standards Institute, CLSI M100-S20, 2007; Evaluation of Lots of Dehydrated Mueller-Hinton Broth for Antimicrobial Susceptibility testing; Proposed Guideline. Wayne, PA. Clinical and Laboratory Standards Institute, CLSI M32-P; Medical microbiology; methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents: agar diffusion test. Berlin, Germany: Deutsches Institut fur Normung, DIN 58940-3: 1989; Medical microbiology -- Methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents-- Part 7: Determination of the minimum bactericidal concentration (MBC) with the aid of microdilution. Berlin, Germany:Deutsches Institut fur Normung, DIN DIN 58940-7: 1994; Part 81: Microdilution; Special requirements for testing of non-fastidious bacteria. Berlin, Germany: Deutsches Institut fur Normung, DIN 58940-81: 2002;Clinical laboratory testing and in vitro diagnostic test systems --- Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices --- Part 1: Reference method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases. London, UK:British Standards Institution, British Standard BS EN ISO 20776-1:2006). Control strain (Aeromonas in Finished Water by Membrane Filtration using Ampicillin-Dextrin Agar with Vancomycin (ADA-V). Washington, DC:Environmental Protection Agency ,EPA EPA Method 1605, 2001).

Supplied as: 
Freeze-dried
Risk group: 
2

Enterococcus faecalis

PTCC No: 
1778
Other collection No. :: 
ATCC 29212, DSM 2570, CIP 103214, LMG 8222, WDCM 00087
Category: 
Quality control strains
Reference strains
Culture condition: 
37°C, Aerobic
Information: 
<- ATCC <- Micro-media System Inc. <- Kaiser-Permanente, strain Portland. Isolated from urine. Quality control strain. Used for food testing, evaluation of Mueller-Hinton agar, media testing, susceptibility disc testing and dilution antimicrobial susceptibility testing. Quality control strain for API, bioMerieux VITEK, IDS, Micro-Media, MicroScan, and Sensititre products. Quality control strain for EUCAST (test for minimum inhibitory concentration of antibiotics).
Literature: 

Control strain (Methodology for the Serum Bactericidal test: Approved Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M21-A, Methods for Determining Bactericidal Activity of Antimicrobial Agents; Approved Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M26-A). Media testing (Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3, Water microbiology. Method2: Culture media, diluents and reagents. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.2-1995, Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003, Medical microbiology-- Culture media -- Part 3: Dip slides for microbiological urine analysis. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58942-3: 2003, 2003, Microbiology of food and animal feeding stuffs --- Horizontal method for the detection and enumeration of Listeria monocytogenes --- Part 1: Detection method, Annex B [BS 5763-18:1997]. London, UK:British Standards Institution;British Standard BS EN ISO 11290-1:1997, Microbiology of food and animal feeding stuffs --- Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli -- Part 3: Most probable number techniques using 5-bromo-4-chloro-3-indolyl-beta-D-glucuronide. London, UK:British Standards Institution;British Standard DD ISO/TS 16649-3:2005, Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003), Quality control of transport medium (Quality Control of Microbiological Transport Systems; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M40-A). Susceptibility disc testing (Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - 9th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M2-A9, Methods for Antimicrobial Disk Susceptibility Testing of Bacteria Isolated from Aquatic Animals. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M42-A, Methods for Broth Dilution Susceptibility Testing of Bacteria Isolated from Aquatic Animals. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M49-A). Susceptibility testing (Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard - 7th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M7-A8, Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M31-A3, Performance Standards for Antimicrobial Susceptibility Testing; Sixteenth Informational Supplement. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M100-S20, 2007, Evaluation of Lots of Dehydrated Mueller-Hinton Broth for Antimicrobial Susceptibility testing; Proposed Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M32-P, Medical microbiology; methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents: agar diffusion test. Berlin, Germany: Deutsches Institut fur Normung; DIN 58940-3: 1989, Medical microbiology -- Methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents-- Part 7: Determination of the minimum bactericidal concentration (MBC) with the aid of microdilution. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58940-7: 1994, 1994, Medical microbiology -- Susceptibility testing of microbial pathogens to antimicrobial agents -- Part 81: Microdilution; Special requirements for testing of non-fastidious bacteria. Berlin, Germany: Deutsches Institut fur Normung; DIN 58940-81: 2002, Clinical laboratory testing and in vitro diagnostic test systems --- Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices --- Part 1: Reference method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases. London, UK:British Standards Institution;British Standard BS EN ISO 20776-1:2006)

Supplied as: 
Freeze-dried
Risk group: 
2

Staphylococcus epidermidis

PTCC No: 
1114
Other collection No. :: 
ATCC 12228, DSM 1798, LMG 10273, CIP 68.21, NCIMB 8853, WDCM 00036
Category: 
Quality control strains
Reference strains
Culture condition: 
37°C, Aerobic
Information: 
<- ATCC <- FDA, strain PCI 1200. Used in food testing, assay of gentamycin, neomycin, netilmycin, novobiocin, paranomycin aminosidine (catenulin) and sisomicin. Applied in Inhibition, media and sterility testing. Quality control strain. Used in Susceptibility disc testing neomycin and vancomycin.
Literature: 

Assay of antibiotics (British Pharmacopoeia Commission Biological assay of antibiotics. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XIV A, 2003. European Pharmacopoeia Commission Microbiological assay of antibiotics. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 2.7.2, 1997. U.S. Pharmacopeia General Chapters; Antibiotics-Microbial Assays. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011. AOAC International Antibiotics in feeds, microbiological methods. Gaithersburg, MD:AOAC International; AOAC "Official Methods of Analysis of the AOAC International" 957.23. Microbial assay for antibiotics. Tokyo, Japan:Japanese Pharmacopoeia;JP JP14e.part I.34. Standard Test Method for Evaluation of Antimicrobial Formulations by the Agar Patch Technique. West Conshohocken, PA:ASTM International; ASTM Standard Test Method E 1882-05). Assay of neomycin (Neomycin in Feeds -- Stahl Microbiological Agar Diffusion Assay. Gaithersburg, MD:AOAC International; AOAC "Official Methods of Analysis of the AOAC International" 998.02). Media testing (Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3. Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media, :International Organization for Standardization/ANSI;ISO ISO 11133-2:2003. ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions). Assay of preservatives (Efficacy of Preservation on Non-Eye Area Water-Miscible Cosmetic and Toiletry Formaulations. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 998.10). Quality control strain for VITEK 2 BCL Comprehensive QC Set. bioMerieux, BD Crystal Rapid Gram-Positive QC Set. BD Becton Dickinson and BD MAX™ StaphSR Assay. BD Becton Dickinson. Virulence genes (Zhang YQ, et al. Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228). Mol. Microbiol. 49: 1577-1593, 2003. PubMed: 12950922)

Supplied as: 
Freeze-dried
Risk group: 
1

Pseudomonas aeruginosa

PTCC No: 
1430
Other collection No. :: 
ATCC 27853, DSM 1117, CCUG 17619, CECT 108, CIP 76.110, JCM 6119, LMG 6395, NCIMB 12469, WDCM 00025
Category: 
Quality control strains
Reference strains
Culture condition: 
37°C, Aerobic
Information: 
<- ATCC <- AA. Madeiros, Boston 41501. Isolated from blood culture. Quality control strain for media testing.Quality control strain for antibiotics susceptibility tests (Susceptibility disc testing). Used in Evaluation of Mueller-Hinton agar and food testing. Quality control strain for identification systems API 20NE and BBL minitek. Used for assay of tobramycin. Quality control strain for Abbott, API, Autobac, BBL, bioMerieux Vitek, Micro-Media, MicroScan®, Roche Diagnostics, Sensititre products, and the Etest Metallo Beta-Lactamase (MBL) strip.
Literature: 

taxonomy (J Gen Microbiol 1982, 128:2945-2954), control strain (Methodology for the Serum Bactericidal test: Approved Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M21-A; Methods for Determining Bactericidal Activity of Antimicrobial Agents; Approved Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M26-A), evaluation of Mueller-Hinton agar (Protocols for Evaluating Dehydrated Mueller-Hinton Agar; Approved Standard. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M6-A2; US General Services Administration Mueller Hinton Medium, dehydrated. Washington, DC:US General Services Administration;Commercial Item Description A-A-50904A, 1986), media testing (Quality Control for Commercially Prepared Microbiological Culture Media; Approved Standard - 3rd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M22-A3;Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 11133-2:2003; Medical microbiology-- Culture media -- Part 3: Dip slides for microbiological urine analysis. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58942-3: 2003; Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution, British Standard DD CEN ISO/TS 11133:2003), quality control strain (API 20 NE. bioMerieux; BD Enterotube II QC Set. BD Becton Dickinson; Enterotube; BD Oxi/Ferm II QC Set. BD Becton Dickinson; Oxi/Ferm; BD Phoenix Gram Negative NMIC and NMIC/ID QC Set. BD Becton Dickinson; Phoenix;MicroScan Dried Gram Negative Panel QC Set. Dade/Siemens; Sensititre Automated Gram Positive ID. Trek Diagnostic Systems; Sensititre MIC Susceptibility. Trek Diagnostic Systems, susceptibility disc testing (Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - 9th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M2-A9; Methods for Antimicrobial Disk Susceptibility Testing of Bacteria Isolated from Aquatic Animals. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M42-A; Methods for Broth Dilution Susceptibility Testing of Bacteria Isolated from Aquatic Animals. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M49-A), susceptibility testing ( Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard - 7th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M7-A8; Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals; Approved Standard - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M31-A3; Performance Standards for Antimicrobial Susceptibility Testing; Sixteenth Informational Supplement. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M100-S20, 2007; Evaluation of Lots of Dehydrated Mueller-Hinton Broth for Antimicrobial Susceptibility testing; Proposed Guideline. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M32-P; Medical microbiology; methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents: agar diffusion test. Berlin, Germany: Deutsches Institut fur Normung, DIN 58940-3: 1989; Medical microbiology -- Methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents-- Part 7: Determination of the minimum bactericidal concentration (MBC) with the aid of microdilution. Berlin, Germany: Deutsches Institut fur Normung, DIN DIN 58940-7: 1994; Medical microbiology: methods for the determination of susceptibility of pathogens (except mycobacteria) to antimicrobial agents; determination of MICs by agar dilution method. Berlin, Germany: Deutsches Institut fur Normung, DIN DIN 58940-6: 1989; Medical microbiology -- Susceptibility testing of microbial pathogens to antimicrobial agents -- Part 81: Microdilution; Special requirements for testing of non-fastidious bacteria. Berlin, Germany: Deutsches Institut fur Normung; DIN 58940-81: 2002; Clinical laboratory testing and in vitro diagnostic test systems --- Susceptibility testing of infectious agents and evaluation of performance of antimicrobial susceptibility test devices --- Part 1: Reference method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases. London, UK:British Standards Institution;British Standard BS EN ISO 20776-1:2006),

Supplied as: 
Freeze-dried
Risk group: 
2

Bacillus cereus

PTCC No: 
1015
Other collection No. :: 
ATCC 11778, PCI 213, NCTC 10320, DSM 345, CIP 64.52, NCIMB 8012, WDCM 00001
Category: 
Quality control strains
Reference strains
Culture condition: 
30 °C, aerobic
Information: 
<- ATCC <- FDA, strain PCI 213; Waksman strain O. Used for assay of tetracycline, chlortetracycline aureomycin, demeclocycline demethylchlortetracycline, doxycycline hydramycin, minocycline and oxytetracycline terramycin. Used for media testing, food testing. Quality control strain.
Literature: 

Antibiotic assay (British Pharmacopoeia Commission Biological assay of antibiotics. London, UK:British Pharmacopoeia Commission; British Pharmacopoeia Appendix XIV A, 2003. AOAC International Antibiotics in feeds, microbiological methods. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 957.23. Simon HJ, Yin EJ. Microbioassay of antimicrobial agents. Appl Microbiol 1970,19: 573-579, PubMed: 4986725). Media testing (Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media.. Geneva (Switzerland): International Organization for Standardization, ANSI;ISO ISO 11133-2:2003.Microbiology of food and animal feeding stuffs-- Guidelines on preparation and production of culture media -- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003. ISO/CD 11133:2009, Annex E. Test microorganisms for commonly used culture media (giving information on the culture medium, culture conditions, test microorganisms, culture collection number of test organisms and the expected reactions)). Quality control strain (Microbiology of food and animal feeding stuffs --- Horizontal method for the determination of low numbers of presumptive Bacillus cereus --- Most probable number technique and detection method. London, UK:British Standards Institution;British Standard BS EN ISO 21871:2006.)

Supplied as: 
Freeze-dried
Risk group: 
2

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