Saccharomyces cerevisiae

PTCC No.: 
5052
Other collection No.: 
ATCC 9763, NRRL Y-567, CBS 2978, DSM 1333, NCTC 10716, NCYC 87, WDCM 00058
Culture condition: 
25 °C
Information: 
<- ATCC <- NRRL. Isolated from distillery yeast. Used for assay of amphotericin B (fungizone), anisomycin, antifongine, candicidin, nystatin (fungicidin), natamycin (pimaricin). Used for media testing, food testing, sterility testing, testing fungicides, susceptibility testing and testing disinfectants. Produces arginase, histone deacetylase, nicotinic acid (niacin) and ethyl alcohol (ethanol). Reference strain for performance testing culture media listed by the ISO TC 34 SC 9 Joint Working Group 5 in the ISO 11133 and by the Working Party on Culture Media of the International Committee on Food Microbiology and Hygiene (ICFMH-WPCM). Recommended as a test microorganism for EP 2.7.2 Microbiological Assays of Antibiotics. Recommended as a test microorganism for JP 16 Monographs. Recommended as a test microorganism for JP 16 Monographs. Recommended as a test microorganism for USP <81> Antibiotics-Microbial Assays. Potential strain for fermented food and beverage production.
Literature: 

Assay of amphotericin (Biological assay of antibiotics, British Pharmacopoeia Appendix XIV A, 2003), amphotericin B fungizone ( U.S. Pharmacopeia General Chapters; <81> ANTIBIOTICS-MICROBIAL ASSAYS, USP28-NF23, 2005), anisomycin (Antibiot Chemother1957, 7: 639-640), antifongine (US Patent 3,052,605 dated Sep 4 1962), candicidin (USP General Chapters 81> ANTIBIOTICS-MICROBIAL ASSAYS; USP28-NF23, 2005), nystatin fungicidin (European Pharmacopoeia EP 2.7.2, 1997; Official Methods of Analysis of the AOAC International" 957.23; Assoc Off Anal Chem 1974, 57: 536-540), Media testing (Microbiology of food and animal feeding stuffs--Guidelines on preparation and production of culture media-- Part 2: Practical guidelines on performance testing of culture media, ISO 11133-2:2003;Microbiology of food and animal feeding stuffs --- Guidelines on preparation and production of culture media --- Part 2: Practical guidelines on performance testing of culture media - Annex B: Recommended test microorganisms for commonly used culture media. London, UK:British Standards Institution;British Standard DD CEN ISO/TS 11133:2003). Production of arginase (Plant Cell Physiol 1973, 14: 641-651), ethanol (J Pharm Sci 1971, 60: 1087-1088; ), histone deacetylase (Biochem J 1991, 303: 723-729), nicotinic acid niacin ( US Patent 3,052,605 dated Sep 4 1962). Sterility testing (J Pharm Sci 1971, 60: 1087-1088). Susceptibility testing (J Clin Microbiol 1994, 32: 1625-1628; Clin Microbiol 1994, 32: 1650-1653; Medical microbiology--Susceptibility testing of microbial pathogens to antimicrobial agents -- Part 84: Microdilution; Special requirements for testing of fungi against antifungal agents. Berlin, Germany: Deutsches Institut fur Normung; DIN 58940-84: 2002). Testing fungicides (Chemical disinfectants and antiseptics -- Quantitative suspension test for the evaluation of fungicidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas -- Test method and requirements (phase 2, step 1), British Standard BS EN 1650:1998; Test method and requirements without mechanical action (phase 2/step 2) British Standard BS EN 13697:2001; Chemical disinfectants and antiseptics - Preservation of test organisms used for the determination of bactericidal, sporicidal and fungicidal activity,British Standard BS EN 12353:2006; Chemical disinfectants and antiseptics --- Application of European Standards for chemical disinfectants and antiseptics.British Standard BS EN 14885:2006). Invertase activity (J Bio. Chem 1980, 255: 6387-6391). Oligosaccharide transfer in microsomes (J Biol Chem 1980,255: 11892-11895). Deoxy-trehalose synthesis (Eur J Biochem 1995, 228: 727-731)

Supplied as: 
Freeze-dried
Risk group: 
1