Modified Reinforced Clostridial Medium

Tryptose……………………………………..10.0 g

Beef Extract…………………………………10.0 g

Yeast Extract………………………………... 3.0 g

Dextrose……………………………………... 5.0 g

NaCl………………………………………….. 5.0 g

Soluble Starch……………………………..... 1.0 g

L-Cysteine HCl…………………………….... 0.5 g

Sodium Acetate……………………………... 3.0 g

DI Water……………………………………..1000 ml

Combine ingredients and dissolve. Adjust pH to 6.8. Dispense and autoclave at 121ºC.