THIOBACILLUS TEPIDARIUS MEDIUM

KH2PO4 4.0 g

K2HPO4 4.0 g

NH4Cl 0.4 g

MgSO4.7H2O 0.8 g

Trace elements (see below) 10.0 ml

K2S4O6 3.0 g

Bromocresol purple (saturated aqu. sol.) 2.0 ml

Bacto-agar (Difco) if needed 10.0 g

Distilled water 1000.0 ml

Final pH: 6.9.

The phosphates are sterilized separately in 1/10 of medium volume and mixed with the other salts when cool. For agar medium autoclave the phosphates (in 100 ml), other salts (in 300 ml), and agar (in 600 ml) separately.

Trace element solution:

Na2 - EDTA 50.0 g

ZnSO4.7H2O 11.0 g

CaCl2.2H2O 7.34 g

MnCl2.4H2O 2.5 g

CoCl2.6H2O 0.5 g

(NH4)6 Mo7O24.4H2O 0.5 g

FeSO4.7H2O 5.0 g

CuSO4.5H2O 0.2 g

NaOH ca 11.0 g

Distilled water 1000.0 ml

Adjust pH to 6.0.