THERMOTOGA MEDIUM

Starch, soluble 5.0 g

KH2PO4 0.5 g

Trace element solution (see below) 15.0 ml

NiCl2.6H2O 2.0 mg

NaCl 20.0 g

Artificial sea water (see below) 250.0 ml

Distilled water 750.0 ml

Yeast extract (Difco) 0.5 g

Resazurin 1.0 mg

Na2S.9H2O 0.5 g

Adjust pH to 6.5. Gas atmosphere: 100% nitrogen.

Artificial sea water:

NaCl 27.7 g

MgSO4.7H2O 7.0 g

MgCl2.6H2O 5.5 g

KCl 0.65 g

NaBr 0.1 g

H3BO3 30.0 mg

SrCl2.6H2O 15.0 mg

Citric acid 10.0 mg

KI 0.05 mg

CaCl2.2H2O 2.25 g

Distilled water 1000.0 ml

Trace element solution:

Nitrilotriacetic acid 1.500 g

MgSO4.7H2O 3.000 g

MnSO4.2H2O 0.500 g

NaCl 1.000 g

FeSO4.7H2O 0.100 g

CoSO4.7H2O 0.180 g

CaCl2.2H2O 0.100 g

ZnSO4.7H2O 0.180 g

CuSO4.5H2O 0.010 g

KAl(SO4)2.12H2O 0.020 g

H3BO3 0.010 g

Na2MoO4.2H2O 0.010 g

NiCl2.6H2O 0.025 g

Na2SeO3.5H2O 0.300 mg

Distilled water 1000.000 ml

First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).