R2A MEDIUM
Medium:
160
Yeast extract 0.50 g
Proteose Peptone 0.50 g
Casamino acids 0.50 g
Glucose 0.50 g
Soluble starch 0.50 g
Na-pyruvate 0.30 g
K2HPO4 0.30 g
MgSO4 x 7 H2O 0.05 g
Agar 15.00 g
Distilled water 1000.00 ml
Adjust pH to 7.2 before adding agar.
Heat medium to dissolve agar, then autoclave at 121℃ for 15 min. The commercial medium is available.