Yeast extract 2.0 g Proteose peptone no. 3 2.0 g Casein peptone, tryptic digested 2.0 g Na2HPO4 x 12 H2O 2.5 g KH2PO4 1.0 g Na-citrate 1.5 g MgSO4 x 7 H2O 0.6 g Glycerol 50.0 ml Tween 0.5 g Agar 15.0 g Distilled water 1000.0 ml
Adjust pH to 7.0.