Thiobacillus medium
Medium:
158
(NH4)2SO4 |
0.10 |
g |
K2HPO4 |
4.00 |
g |
KH2PO4 |
4.00 |
g |
MgSO4 x 7 H2O |
0.10 |
g |
CaCl2 |
0.10 |
g |
FeCl3 x 6 H2O |
0.02 |
g |
MnSO4 x H2O |
0.02 |
g |
Na2S2O3 x 5 H2O |
10.00 |
g |
Distilled water |
1000.00 |
ml |
Dissolve ingredients except thiosulfate and adjust the pH to 6.6. Dispense in suitable cultivation vials and autoclave. Add thiosulfate to the autoclaved medium from a sterile stock solution.