Thiobacillus medium

Medium: 
158

  (NH4)2SO4

0.10

g

  K2HPO4

4.00

g

  KH2PO4

4.00

g

  MgSO4 x 7 H2O

0.10

g

  CaCl2

0.10

g

  FeCl3 x 6 H2O

0.02

g

  MnSO4 x H2O

0.02

g

  Na2S2O3 x 5 H2O

10.00

g

  Distilled water

1000.00

ml

 

Dissolve ingredients except thiosulfate and adjust the pH to 6.6. Dispense in suitable cultivation vials and autoclave. Add thiosulfate to the autoclaved medium from a sterile stock solution.