HETEROTOTROPHIC NITROBACTER MEDIUM

Yeast extract 1.50 g

Peptone 1.50 g

Na-pyruvate 0.55 g

Trace element solution 1.00 ml

Stock solution 100.00 ml

Distilled water 899.00 ml

Adjust pH to 7.4 with NaOH or KOH.

Stock solution:

CaCO3 0.07 g

NaCl 5.00 g

MgSO4.7H2O 0.50 g

KH2PO4 1.50 g

Distilled water 1000.00 ml

Trace element solution:

MnSO4.H2O 33.80 mg

H3BO3 49.40 mg

ZnSO4.7H2O 43.10 mg

(NH4)6Mo7O24 37.10 mg

FeSO4.7H2O 97.30 mg

CuSO4.5H2O 25.00 mg

Distilled water 1000.00 ml