AZOTOBACTER BASAL MEDIUM

K2HPO4 1.0 g

MgSO4 2.2 g

NaCl 0.2 g

FeSO4 0.005 g

Soil extract 100.0 ml

Agar 15.0 g

Tap Water 900.0 ml

Soil extract:

African Violet soil 77.0 g

Na2CO3 0.2 g

Distilled Water 200.0 ml

Autoclave soil suspension for one hour at 121°C. To obtain a clear supernatant allow to settle and centrifuge. Adjust pH to 7.2.