MEDIUM BG-11 FOR BLUE-GREEN ALGAE

NaNO3 1.5 g

K2HPO4 0.04 g

MgSO4 . 7H2O 0.075 g

CaCl2 . 2H2O 0.036 g

Citric acid 6.0 mg

Ferric ammonium citrate 6.0 mg

EDTA 1.0 mg

Na2CO3 0.02 g

Trace Metal Mix A5 (see below) 1.0 ml

Agar (if needed) 10.0 g

Distilled water 1.0 L

Adjust final pH to 7.1.

Autoclave at 121°C for 15 minutes.

Trace Metal Mix A5:

H3BO3 2.86 g

MnCl2 . 4H2O 1.81 g

ZnSO4 . 7H2O 0.222 g

Na2MoO4 . 2H2O 0.39 g

CuSO4 . 5H2O 0.079 g

Co(NO3)2 . 6H2O 49.4 mg

Distilled water 1.0 L