Yeast extract 5.00 g
Casamino acids 5.00 g
Na-glutamate 1.00 g
KCl 2.00 g
Na3-citrate 3.00 g
MgSO4.7H2O 20.00 g
NaCl 200.0 g
FeCl2.4H2O 36.00 mg
MnCl2.4H2O 0.36 mg
Agar 20.00 g
Add distilled water to give a final volume of 1000 ml.
Adjust pH to 7.0 - 7.2