AZOTOBACTER MEDIUM

Glucose 5.00 g

Mannitol 5.00 g

CaCl2.H2O 0.10 g

MgSO4.7H2O 0.10 g

Na2MoO4.2H2O 5.00 mg

K2HPO4 0.90 g

KH2PO4 0.10 g

FeSO4.7H2O 0.01 g

CaCO3 5.00 g

Agar 15.00 g

Distilled water 950.00 ml

Adjust pH to 7.3.

Sterilize glucose and mannitol separately (in 50 ml H2O) and add to the medium after autoclaving.