ACIDIANUS BRIERLEYI MEDIUM

(NH4)2SO4 3.00 g

K2HPO4.3 H2O 0.50 g

MgSO4.7 H2O 0.50 g

KCl 0.10 g

Ca(NO3)2 0.01 g

Yeast extract 0.20 g

Sulfur (flowers) 10.00 g

Distilled water 1000.00 ml

Dissolve ingredients except yeast extract and sulfur. Adjust pH with 6 N H2SO4 to 1.5 - 2.5. Yeast extract (10% w/v in distilled water) is autoclaved separately. Sulfur is sterilized by steaming for 3 hours on each of 3 successive days.