MS-MEDIUM

MS-Medium

(NH4)2SO4 2.00 g

MgSO4.7H2O 0.25 g

K2HPO4 0.10 g

KCl 0.10 g

Distilled water 1000.00 ml

Adjust pH of MS-Medium to 3.5 with 1N sulfuric acid prior to autoclaving. Before use, add 5g/l sterile powdered sulfur.

Sterilize powdered elemental sulfur by steaming for 3 hours at 100°C on three successive days.