Agar 20.0 g
Soluble Starch 10.0 g
CaCO3 2.0 g
(NH4) 2SO4 2.0 g
K2HPO4 1.0 g
MgSO4.7H2O 1.0 g
NaCl 1.0 g
FeSO4.7H2O 1.0 mg
MnCl2.7H2O 1.0 mg
ZnSO4.7H2O 1.0 mg
Distilled water 1.0 L
pH 7.2 ± 0.2 at 25°C
ISP Medium 4 (Difco 277210)
