NaNO3 3.0 g
K2HPO4 1.0 g
MgSO4 .7H2O 0.5 g
KCl 0.5 g
FeSO4.7H2O 0.01 g
Agar 15.0 g
DI Water 900.0 ml
Autoclave at 121°C
Let cool and add following filter sterilized solution:
Sucrose (commercial grade) 30 g
DI Water 100 ml
Final pH 7.3 ± 0.2 at 25°C
