Alicyclobacillus medium (BAT medium)

Solution A:

CaCl2 x 2 H2O 0.25 g

MgSO4 x 7 H2O 0.50 g

(NH4)2 SO4 0.20 g

Yeast extract 2.00 g

Glucose 5.00 g

KH2PO4 3.00 g

Distilled water (for liquid medium) 1000.00 ml

Distilled water (for solid medium) 500.00 ml

Adjust pH to 4.0

Solution B:

Trace element sol. SL-6 1.00 ml

Trace element solution SL-6:

ZnSO4 . 7 H2O 0.10 g

MnCl2 . 4 H2O 0.03 g

H3BO3 0.30 g

CoCl2 . 6 H2O 0.20 g

CuCl2 . 2 H2O 0.01 g

NiCl2 . 6 H2O 0.02 g

Na2MoO4 . 2 H2O 0.03 g

Distilled water 1000.00 ml

Solution C:

Agar 15.00 g

Distilled water 500.00 ml

Sterilize separately. For liquid medium combine solution A (with1000.0 ml distilled water) and solution B. For solid medium combine solution A (with 500 ml distilled water), solution B and solution C. For strains of A. cycloheptanicus add 5 g/l of yeast extract instead of 2 g/l.