Mannitol 10.0 gCaCO3 4.0 gKH2PO4 0.5 gYeast extract 0.4 gMgSO4.7H2O 0.2 gNaCl 0.1 gAgar 15.0 gDistilled water 1000.0 mlpH 6.8 ± 0.2 at 25°C