Peptone 10.0 g
Yeast extract 5.0 g
Glucose 10.0 g
K2HPO4 1.0 g
Agar 15.0 g
Distilled water 900.0 ml
Autoclave at 115C for 15 minutes. Cool to 50C and add the following filter-sterilized solution:
10% Na2CO3 100.0 ml
