9K MEDIUM

Medium: 
106

Solution 1:

(NH4)2SO4                 3.0 g

KCl                             0.1 g

K2HPO4                      0.5 g

MgSO4.7H2O             0.5 g

Ca(NO3)2                 0.01 g

Distilled Water      500.0 ml.

 

Solution 2:

FeSO4.7H2O         44.22 g

H2SO4                     1.0 ml

Distilled Water        250 ml

 

Solution 3:

Agar                         15.0 g

Distilled Water          250 ml

 

Sterilize solution 1 and 3 with autoclave at 121 °C for 15 minutes. Filter sterilize solution 2 and mix  three solutions. Then adjust pH to 2-2.3.

 

For broth medium omit agar and add 250 ml distilled water to solution 1.