THIOBACILLUS THYASIRIS and T. HALOPHILUS MEDIUM

Use medium 65 with 25.1 g of NaCl, added to medium part B,and with 1% v/v of filter-sterilized vitamin mixture. Medium pH 7.3-7.6.

Vitamin mixture per liter:

Thiamine-HCl.2H2O 10.0 mg

Nicotinic acid 20.0 mg

Pyridoxine-HCl 20.0 mg

p-Aminobenzoic acid 10.0 mg

Riboflavin 20.0 mg

Ca-pantothenate 20.0 mg

Biotin 1.0 mg

Vitamin B12 1.0 mg

Adjust pH to 7.0