NUTRIENT AGAR

Medium: 
2

Peptone 5.0 g

Meat extract 3.0 g

Agar 15.0 g

Distilled water 1.0 L

Adjust pH to 7.0.

For sporulation of Bacillus strains the addition of 10.0 mg MnCl2.2H2O, 100 mg CaCl2.2H2O, and 500 mg MgSo4.7H2o is recommended.